초록
<P><B>Abstract</B></P> <P>Biohydrogen is one of the alternative energy that has a potential to be convenient future green energy. The supremacy of using H<SUB>2</SUB> is that the only product of O<SUB>2</SUB> and H<SUB>2</SUB> reaction is water without carbon dioxide. H<SUB>2</SUB> can be produced by biological dark-fermentative hydrogen-producing bacteria. <I>Bacillus</I> is one of efficient hydrogen producer belonged to the division of Firmicutes which is related to highly-effective hydrogen producer <I>Clostridium</I> species. In this research, the new isolate of <I>Bacillus coagulans</I> is isolated from molasses from bioethanol plant in Kanchanaburi Province. Reducing sugar, sucrose and glucose were analyzed in molasses results sugar content 0.61, 3.0, and 0.08 g/100g, respectively. In this research, <I>B. coagulans</I> is focused on the ability to convert sugar into hydrogen. It is a gram positive, rod-shape bacterium that known to be an ideal biocatalyst for lignocellulosic biomass fermentation for biofuels production. Gas production were observed after 168 hr fermentation in modified sucrose-based synthetic medium. The isolate produces hydrogen gas 1.634 mol H<SUB>2</SUB>/mol hexose (48 mL in 30 mL synthetic medium supplemented with 15 g/L sucrose). Soluble metabolite such as volatile fatty acid (VFA) are observed.</P>