BioChem - DOS

Improvement and modeling of culture parameters to enhance biomass and lipid production by the oleaginous yeast Cryptococcus curvatus grown on acetate

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Beligon, V.; Poughon, L.; Christophe, G.; Lebert, A.; Larroche, C.; Fontanille, P.

The improvement of culture parameters for lipid production from acetate as carbon source was investigated using the oleaginous yeast Cryptococcus curvatus. A new pH regulation system dispensing acetate was developed for fed-batch culture and allowed obtaining nearly 80g/L biomass within 60h with a maximal growth rate of 0.28h<SUP>-1</SUP>. A biological model was developed from experimental data. The influence of three C/N ratios of 300, 500 and 900 were tested during a multi-phases process on lipid accumulation. The C/N ratio of 300 was reported to be the most suitable for lipid storage. No significant increase of lipids content was obtained with higher value. A maximal content of 60% DCW of lipid was obtained. The determination of fatty acids profiles of the microbial oils has confirmed that the valorization of acetate by microbial oils production was a promising perspective.

2015

Elsevier Applied Science

0960-8524

192

pp.582-591

10.1016/j.biortech.2015.06.041

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72336323

Volatile fatty acids; Cryptococcus curvatus; Microbial lipids; Bioconversion; Biodiesel