Enzymatic synthesis of rare sugars with L-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8

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논문

Enzymatic synthesis of rare sugars with L-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8

학술지

Bioorganic & medicinal chemistry letters

저자명

Li, Z.; Wu, X.; Cai, L.; Duan, S.; Liu, J.; Yuan, P.; Nakanishi, H.; Gao, X.D.

초록

l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaD<SUB>T.mari</SUB>) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.

발행연도

2015

발행기관

Pergamon Press

ISSN

0960-894x

ISSN

1464-3405

권

호

페이지

pp.3980-3983