초록
<P>A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera <I>Rhodosporidium</I>, <I>Rhodotorula</I>, and <I>Cryptococcus</I>. Two representative strains <I>Rhodosporidium</I> TJUWZ4 and <I>Cryptococcus</I> TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that <I>Rhodosporidium</I> TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation, <I>Rhodosporidium</I> TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13568-017-0329-x) contains supplementary material, which is available to authorized users.</P>