BioChem - DOS

Chemoenzymatic cascade synthesis of optically pure alkanoic acids by using engineered arylmalonate decarboxylase variants

Chemistry : a European journal

Enoki, Junichi; Mü gge, Carolin; Tischler, Dirk; Miyamoto, Kenji; Kourist, Robert

<P><B>Abstract</B></P><P>Arylmalonate decarboxylase (AMDase) catalyzes the cofactor&#8208;free asymmetric decarboxylation of prochiral arylmalonic acids and produces the corresponding monoacids with rigorous <I>R</I> selectivity. Alteration of catalytic cysteine residues and of the hydrophobic environment in the active site by protein engineering has previously resulted in the generation of variants with opposite enantioselectivity and improved catalytic performance. The substrate spectrum of AMDase allows it to catalyze the asymmetric decarboxylation of small methylvinylmalonic acid derivatives, implying the possibility to produce short&#8208;chain 2&#8208;methylalkanoic acids with high optical purity after reduction of the nonactivated C=C double bond. Use of diimide as the reductant proved to be a simple strategy to avoid racemization of the stereocenter during reduction. The developed chemoenzymatic sequential cascade with use of <I>R</I>&#8208; and <I>S</I>&#8208;selective AMDase variants produced optically pure short&#8208;chain 2&#8208;methylalkanoic acids in moderate to full conversion and gave both enantiomers in excellent enantiopurity (up to 83 % isolated yield and 98 % <I>ee</I>).</P>

2019

cc-by

0947-6539

1521-3765

25

19

pp.5071-5076

10.1002/chem.201806339

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART95997695

arylmalonate decarboxylase; biocatalysis; cascade reactions; enzymes; hydrogenation