BioChem - DOS

A novel ginsenoside-hydrolyzing enzyme from Penicillium oxalicum and its application in ginsenoside Rd production

Biocatalysis and biotransformation

Gao, Juan; Hu, Yanbo; Ji, Li; Wang, Nan; Wang, Jiao; Tai, Guihua; Zhou, Yifa

<P>The fungus <I>Penicillum oxalicum</I> can selectively metabolize the major 20(S)-protopanaxadiol ginsenosides Rb1, Rb2, and Rc using extracellular glycosidases yielding a series of bioactive metabolites. A &beta;-glucosidase GH1 was purified from the culture of <I>P. oxalicum</I> with a yield of 9.5% and a specific activity of 3.9 &times; 10<SUP>3</SUP> U/mg. GH1 was a tetramer with a native molecular weight of 484 kDa and its <I>p</I>I value was pH 4.2. GH1 specifically cleaved the &beta;-(1-6)-glucosidic linkage at C-20 site of ginsenoside Rb1 to give the sole product Rd. The optimum conditions were established to be pH 4.5, 55&deg;C, and 0.25 U/ml purified enzyme at 2 mg/ml ginsenoside Rb1. GH1 could be used in the pharmaceutical industry.</P>

2013

Informa Healthcare

1024-2422

1029-2446

31

6

pp.305-312

10.3109/10242422.2013.857316

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68339228

Ginsenoside Rb1; ginsenoside Rd; β-glucosidase; Penicillium oxalicum; biotransformation