초록
This study deals with the effects of initial inoculum density and aeration volume on biomass and bioactive compound production in adventitious roots of Eleutherococcus koreanum Nakai in bulb-type bubble bioreactors (3-L capacity). While the fresh and dry weights of the roots increased with increasing inoculum density, the highest percentage dry weight and accumulation of total target compounds (eleutheroside B and E, chlorogenic acid, total phenolics, and flavonoids) were noted at an inoculum density of 5.0gL<SUP>-1</SUP>. Poor aeration volume (0.05vvm) stunted root growth, and high aeration volume (0.4vvm) caused physiological disorders. Moreover, an inoculum density of 5.0gL<SUP>-1</SUP> and an aeration volume of 0.1vvm resulted in the highest concentration of total target compounds and least root death. Such optimization of culture conditions will be beneficial for the large-scale production of E. koreanum biomass and bioactive compounds.