BioChem - DOS

Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol

Journal of biotechnology

Hughes, Stephen R.; Moser, Bryan R.; Robinson, Samantha; Cox, Elby J.; Harmsen, Amanda J.; Friesen, Jon A.; Bischoff, Kenneth M.; Jones, Marjorie A.; Pinkelman, Rebecca; Bang, Sookie S.; Tasaki, Ken; Doll, Kenneth M.; Qureshi, Nasib; Liu, Siqing; Saha, Badal C.; Jackson, John S.; Cotta, Michael A.; Rich, Joseph O.; Caimi, Paolo

<P><B>Highlights</B></P><P>&#x025BA; Gene ORF for truncated <I>Candida antarctica</I> lipase B (CALB) was expressed in yeast. &#x025BA; Most abundant synthetic CALB contained amino acids 35&ndash;317 of full-length lipase. &#x025BA; Resin-bound CALB used to produce ethyl and n-butyl esters from corn and soybean oils. &#x025BA; Ethyl ester yield was 4-fold greater from corn oil than from soybean oil. &#x025BA; Ratio 5:1 (v/v) ethanol:corn oil optimal; butyl 56% higher than ethyl ester production.</P> <P><B>Abstract</B></P><P>A gene encoding a synthetic truncated <I>Candida antarctica</I> lipase B (CALB) was generated via automated PCR and expressed in <I>Saccharomyces cerevisiae</I>. Western blot analysis detected five truncated CALB variants, suggesting multiple translation starts from the six in-frame ATG codons. The longest open reading frame, which corresponds to amino acids 35&ndash;317 of the mature lipase, appeared to be expressed in the greatest amount. The truncated CALB was immobilized on Sepabeads<SUP>&reg;</SUP> EC-EP resin and used to produce ethyl and butyl esters from crude corn oil and refined soybean oil. The yield of ethyl esters was 4-fold greater from corn oil than from soybean oil and was 36% and 50% higher, respectively, when compared to a commercially available lipase resin (Novozym 435) using the same substrates. A 5:1 (v/v) ratio of ethanol to corn oil produced 3.7-fold and 8.4-fold greater yields than ratios of 15:1 and 30:1, respectively. With corn oil, butyl ester production was 56% higher than ethyl ester production. Addition of an ionic catalytic resin step prior to the CALB resin increased yields of ethyl esters from corn oil by 53% compared to CALB resin followed by ionic resin. The results suggest resin-bound truncated CALB has potential application in biodiesel production using biocatalysts.</P>

2012

Elsevier

0168-1656

1873-4863

159

1

pp.69-77

10.1016/j.jbiotec.2012.01.025

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62210267

Truncated Candida antarctica lipase B; Transesterification; Resin-supported biocatalyst; Sepabeads®