BioChem - DOS

Comparison of the synergistic action of two thermostable xylanases from GH families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zhang, Junhua; Tuomainen, Pä ivi; Siika-aho, Matti; Viikari, Liisa

<P><B>Highlights</B></P><P>&#x025BA; GH10 xylanase showed better hydrolytic capacity than GH11 xylanase in wheat straw hydrolysis. &#x025BA; Clear synergy interactions between thermostable xylanases and cellulases were demonstrated. &#x025BA; Excellent hydrolytic properties of the thermostable xylanase were observed at high temperature. &#x025BA; The activities from the host strain <I>Trichoderma reesei</I> had a beneficial contribution to hydrolysis.</P> <P><B>Abstract</B></P><P>Recombinant xylanase preparations from <I>Nonomuraea flexuosa</I> (Nf Xyn, GH11) and <I>Thermoascus aurantiacus</I> (Ta Xyn, GH10) were evaluated for their abilities to hydrolyze hydrothermally pretreated wheat straw. The GH family 10 enzyme Ta Xyn was clearly more efficient in solubilizing xylan from pretreated wheat straw. Improvement of the hydrolysis of hydrothermally pretreated wheat straw by addition of the thermostable xylanase preparations to thermostable cellulases was evaluated. Clear synergistic enhancement of hydrolysis of cellulose was observed when cellulases were supplemented even with a low amount of pure xylanases. Xylobiose was the main hydrolysis product from xylan. It was found that the hydrolysis of cellulose increased nearly linearly with xylan removal during the enzymatic hydrolysis. The results also showed that the xylanase preparation from <I>T. aurantiacus</I>, belonging to GH family 10 always showed better hydrolytic capacity of solubilizing xylan and acting synergistically with thermostable cellulases in the hydrolysis of hydrothermally pretreated wheat straw.</P>

2011

0960-8524

102

19

pp.9090-9095

10.1016/j.biortech.2011.06.085

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57231271

Xylanase; Enzymatic hydrolysis; Wheat straw; Cellulase; Thermostable