초록
<P><B>Abstract</B></P> <P>In this study, the xylanase gene from <I>Cellulomonas flavigena</I> KCTC 9104 was cloned into pPICZαB and expressed in <I>Pichia pastoris</I> X-33. An extracellular <I>endo</I>-1,4-<I>β</I>-xylanase was produced by novel engineered <I>P. pastoris</I> (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38kDa molecular weight, 55°C optimum temperature and optimum pH of 6. Under the conditions, the <I>K<SUB>m</SUB> </I> and <I>V</I> <SUB>max</SUB> of rXynCf were 3.6±0.08mg/mL and 4505±52μmol/minmg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An extracellular xylanase was produced from a novel engineered <I>P. pastoris</I> (rXynCf). </LI> <LI> Biochemical characterization of rXynCf was performed. </LI> <LI> Empty fruit bunch (EFB) was selected as a feedstock for bioconversion process. </LI> <LI> rXynCf was applied in the hydrolysis process to produce sugars from EFB. </LI> <LI> rXynCf achieved 95.5% sugars conversion compared with commercial xylanase. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>