BioChem - DOS

Improving the Performance of Methanol Biofuel Cells Utilizing an Enzyme Cascade Bioanode with DNA-Bridged Substrate Channeling

ACS energy letters

Xia, Lin; Van Nguyen, Khiem; Holade, Yaovi; Han, Han; Dooley, Kevin; Atanassov, Plamen; Banta, Scott; Minteer, Shelley D.

<P>The development of enzymatic biofuel cells has been plagued by the high cost of enzyme purification and low efficiency of fuel oxidation. Here, we demonstrate a protein purification-free approach to assemble an alcohol dehydrogenase and aldehyde dehydrogenase enzyme cascade-based bioanode for use in a methanol biofuel cell. Each enzyme was fused to a different sequence-specific zinc finger DNA-binding protein. The zinc finger domains serve as both tags to isolate the enzymes from crude cell lysates as well as anchors to immobilize the enzymes on DNA-modified multiwalled carbon nanotubes. The biofuel cells based on the enzyme cascade bioanodes show a maximum power output of 24.5 &plusmn; 3.2 &mu;W cm<SUP>&ndash;2</SUP>, which is comparable to fuel cells utilizing purified enzymes. Further analysis of kinetic behavior revealed a significant increase in the reactivity of the complexes due to substrate channeling of the aldehyde intermediate.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aelccp/2017/aelccp.2017.2.issue-6/acsenergylett.7b00134/production/images/medium/nz-2017-001349_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nz7b00134'>ACS Electronic Supporting Info</A></P>

2017

American Chemical Society

2380-8195

2

6

pp.1435-1438

10.1021/acsenergylett.7b00134.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77967558

https://doi.org/10.1021/acsenergylett.7b00134.s001