초록
Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett-Burman design and the Face Centered Central Composite Design (FCCCD). Plackett-Burman experimental design was used to screen the medium components and process conditions. It was found that carboxymethylcellulose (CMC), FeSO<SUB>4</SUB>.7H<SUB>2</SUB>O, NaCl, MgSO<SUB>4</SUB>.7H<SUB>2</SUB>O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase. On the other hand, KH<SUB>2</SUB>PO<SUB>4</SUB>, KOH, yeast extract and temperature had a negative effect on enzyme production. Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029U/ml to 0.0625U/ml, which was approximately 2.2-times greater than before optimization.