초록
<P><B>Abstract</B></P> <P> <I>Clostridium beijerinckii</I> CC101 was engineered to overexpress aldehyde/alcohol dehydrogenase (<I>adh</I>E2) and CoA-transferase (<I>ctf</I>AB). Solvent production and acid assimilation were compared between the parental and engineered strains expressing only <I>adh</I>E2 (CC101-SV4) and expressing <I>adh</I>E2, <I>ald</I> and <I>ctfAB</I> (CC101-SV6). CC101-SV4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6g/L. Compared to CC101, CC101-SV6 produced more butanol (∼12g/L) from glucose and was able to re-assimilate more acids, which prevented “acid crash” and increased butanol production, under all conditions studied. CC101-SV6 also showed better ability in using glucose and xylose present in sugarcane bagasse hydrolysate, and produced 9.4g/L solvents (acetone, butanol and ethanol) compared to only 2.6g/L by CC101, confirming its robustness and better tolerance to hydrolysate inhibitors. The engineered strain of <I>C. beijerinckii</I> overexpressing <I>adh</I>E2 and <I>ctf</I>AB should have good potential for producing butanol from lignocellulosic biomass hydrolysates.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>C. beijerinckii</I> was engineered to overexpress <I>adh</I>E2 and <I>ctf</I>AB. </LI> <LI> Mutant showed greater ability for acids assimilation, which prevented acid crash. </LI> <LI> Increased acids assimilation also increased butanol production. </LI> <LI> Mutant also showed robustness in resisting sugarcane bagasse hydrolysate inhibitors. </LI> <LI> It has good potential for biobutanol production from lignocellulosic biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>