BioChem - DOS

Effect of sodium accumulation on heterotrophic growth and polyhydroxybutyrate (PHB) production by Cupriavidus necator

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Mozumder, Md.S.I.; Garcia-Gonzalez, L.; De Wever, H.; Volcke, E.I.P.

This study evaluates the effect of sodium (Na<SUP>+</SUP>) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na<SUP>+</SUP>: biomass growth became zero at 8.9g/L Na<SUP>+</SUP> concentration while PHB production was completely stopped at 10.5g/L Na<SUP>+</SUP>. A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na<SUP>+</SUP> inhibition effect. The parameters related to Na<SUP>+</SUP> inhibition were estimated based on shake flask experiments. The accumulated Na<SUP>+</SUP> showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na<SUP>+</SUP> due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.

2015

Elsevier Applied Science

0960-8524

191

pp.213-218

10.1016/j.biortech.2015.04.110

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72168968

Na+ inhibition; Biomass growth; Polyhydroxybutyrate (PHB) production; Mathematical modeling; Simulation