초록
<P><B>Background and objective</B></P><P>Impaired gut barrier contributes to the progression of type 2 diabetes mellitus (T2DM), and the gut microbiota and metabolome play an important role in it. Hemicellulose, a potential prebiotics, how its supplementation impacted the glucose level, the impaired gut barrier, and the gut microbiota and metabolome in T2DM remained unclear.</P><P><B>Methods</B></P><P>In this study, some mice were arranged randomly into four groups: db/db mice fed by a compositionally defined diet (CDD), db/db mice fed by a CDD with 10% and 20% hemicellulose supplementation, and control mice fed by a CDD. Body weight and fasting blood glucose levels were monitored weekly. The gut barrier was evaluated. Fresh stool samples were analyzed using metagenomic sequencing and liquid chromatography-mass spectrometry to detect gut microbiota and metabolome changes. Systemic and colonic inflammation were evaluated.</P><P><B>Results</B></P><P>Better glycemic control, restoration of the impaired gut barrier, and lowered systemic inflammation levels were observed in db/db mice with the supplementation of 10 or 20% hemicellulose. The gut microbiota showed significant differences in beta diversity among the four groups. Fifteen genera with differential relative abundances and 59 significantly different metabolites were found. In the db/db group, hemicellulose eliminated the redundant <I>Faecalibaculum</I> and <I>Enterorhabdus</I>. The increased succinate and ursodeoxycholic acid (UDCA) after hemicellulose treatment were negatively correlated with <I>Bifidobacterium, Erysipelatoclostridium</I>, and <I>Faecalibaculum</I>. In addition, hemicellulose reduced the colonic expressions of TLR2/4 and TNF-α in db/db mice.</P><P><B>Conclusion</B></P><P>Hemicellulose may serve as a potential therapeutic intervention for T2DM by improving impaired intestinal mucosal barrier integrity, modulating gut microbiota composition, and altering the metabolic profile.</P>