초록
<P><B>Abstract</B></P> <P>A new isolated <I>Schizochytrium</I> sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06g/L was obtained by feeding 15g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74g/L and 241.5mg/L/h, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Schizochytrium</I> sp. LU310 is an ideal stain for DHA-producing as its impressive productivity. </LI> <LI> Baffled flask enhanced in high cell density cultivation of DHA. </LI> <LI> MSG-feeding combined with glucose feeding strategy was extremely useful for DHA production. </LI> <LI> DHA production was linearly correlated with the concentrations of carbon and nitrogen. </LI> </UL> </P>