초록
<P>Instead of β-cyclodextrin (β-CD), branched β-CDs have been increasingly used in many aspects as they possess better solubility and higher bioadaptability. But most commercialized branched β-CDs were chemically synthesized. Thus, the glucosyl-β-cyclodextrin (G<SUB>1</SUB>-β-CD) prepared via enzymatic approach could be a nice substitute. However, the yield of G<SUB>1</SUB>-β-CD was low. Here, we reported a controlled two-step reaction to efficiently prepare G<SUB>1</SUB>-β-CD from maltodextrins by β-cyclodextrin glucosyltransferase (β-CGTase) and amyloglucosidase (AG). Compared to the single β-CGTase reaction, controlled two-step reaction caused a yield increase of G<SUB>1</SUB>-β-CD by 130%. Additionally, the percentage of G<SUB>1</SUB>-β-CD was enhanced from 2.4% to 24.0% and the side products α-CD and γ-CD were hydrolyzed because of the coupling activity of β-CGTase. Thus, this controlled two-step reaction might be an efficient approach for industrial production of pure G<SUB>1</SUB>-β-CD.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-29/acs.jafc.7b02079/production/images/medium/jf-2017-020795_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf7b02079'>ACS Electronic Supporting Info</A></P>