Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

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논문

Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Khramtsov, N.; McDade, L.; Amerik, A.; Yu, E.; Divatia, K.; Tikhonov, A.; Minto, M.; Kabongo-Mubalamate, G.; Markovic, Z.; Ruiz-Martinez, M.; Henck, S.

초록

In this study an industrial Saccharomyces cerevisiae yeast strain capable of fermenting ethanol from pretreated lignocellulosic material was engineered. Genes encoding cellulases (endoglucanase, exoglucanase and β-glucosidase) were integrated into the chromosomal ribosomal DNA and delta regions of a derivative of the K1-V1116 wine yeast strain. The engineered cellulolytic yeast produces ethanol in one step through simultaneous saccharification and fermentation of pretreated biomass without the addition of exogenously produced enzymes. When ethanol fermentation was performed with 10% dry weight of pretreated corn stover, the recombinant strain fermented 63% of the cellulose in 96h and the ethanol titer reached 2.6% v/v. These results demonstrate that cellulolytic S. cerevisiae strains can be used as a platform for developing an economical advanced biofuel process.

발행연도

2011

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

102

호

페이지

pp.8310-8313

DOI