초록
Acetone butanol ethanol (ABE) was produced from enzymatic-hydrolyzed corncobs by Clostridium saccharobutylicum DSM 13864. Pretreatment of corncobs was carried out with 0.5 mol L<SUP>-1</SUP> NaOH followed by enzymatic hydrolysis. The yield of total reducing sugars was 917 g kg<SUP>-1</SUP> pretreated (de-lignified) and washed corncobs. The hydrolysate was used without sediments removal for ABE fermentation. A solvent production of 19.44 g L<SUP>-1</SUP> with 12.27 g L<SUP>-1</SUP> butanol was obtained from 55.22 g L<SUP>-1</SUP> sugars, resulting in an ABE yield of 350 g kg<SUP>-1</SUP> and a production rate of 0.54 g L<SUP>-1</SUP> h<SUP>-1</SUP>. A control experiment using 55.3 g L<SUP>-1</SUP> mixed sugars resulted in an ABE production of 16.81 g L<SUP>-1</SUP> with 10.26 g L<SUP>-1</SUP> butanol, corresponding to an ABE yield of 300 g kg<SUP>-1</SUP> and a production rate of 0.47 g L<SUP>-1</SUP> h<SUP>-1</SUP>, indicating that the enzymatic hydrolysates may contain stimulating compounds that can improve the ABE fermentation.