BioChem - DOS

Genome Shuffling of Clostridium acetobutylicum CICC 8012 for Improved Production of Acetone-Butanol-Ethanol (ABE)

Current microbiology

Gao, Xiaofeng; Zhao, Hai; Zhang, Guohua; He, Kaize; Jin, Yanling

<P>Genome shuffling was applied to increase ABE production of the strict anaerobe C. acetobutylicum CICC 8012. By using physical and chemical mutagenesis, strains with superior streptomycin sulfate, 2-deoxy-D-glucose and butanol tolerance levels were isolated. These strains were used for genome shuffling. The best performing strain F2-GA was screened after two rounds of genome shuffling. With 55 g glucose/l as carbon source, F2-GA produced 22.21 g ABE/l in 72 h and ABE yield reached 0.42 g/g which was about 34.53 % improvement compared to the wild type. Fermentation parameters and gene expression of several key enzymes in ABE metabolic pathways were varied significantly between F2-GA and the wild type. These results demonstrated the potential use of genome shuffling to microbial breeding which were difficult to deal with traditional methods.</P>

2012

Springer-Verlag

0343-8651

1432-0991

65

2

pp.128-132

10.1007/s00284-012-0134-3

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63392894