BioChem - DOS

Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

Scientific reports

Liu, Zhuo; Ho, Shih-Hsin; Sasaki, Kengo; den Haan, Riaan; Inokuma, Kentaro; Ogino, Chiaki; van Zyl, Willem H.; Hasunuma, Tomohisa; Kondo, Akihiko

<P>Cellulosic biofuel is the subject of increasing attention. The main obstacle toward its economic feasibility is the recalcitrance of lignocellulose requiring large amount of enzyme to break. Several engineered yeast strains have been developed with cellulolytic activities to reduce the need for enzyme addition, but exhibiting limited effect. Here, we report the successful engineering of a cellulose-adherent <I>Saccharomyces cerevisiae</I> displaying four different synergistic cellulases on the cell surface. The cellulase-displaying yeast strain exhibited clear cell-to-cellulose adhesion and a “tearing” cellulose degradation pattern; the adhesion ability correlated with enhanced surface area and roughness of the target cellulose fibers, resulting in higher hydrolysis efficiency. The engineered yeast directly produced ethanol from rice straw despite a more than 40% decrease in the required enzyme dosage for high-density fermentation. Thus, improved cell-to-cellulose interactions provided a novel strategy for increasing cellulose hydrolysis, suggesting a mechanism for promoting the feasibility of cellulosic biofuel production.</P>

2016

Nature Publishing Group

cc-by

2045-2322

6

pp.24550

10.1038/srep24550

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75372329

https://www.nature.com/articles/srep24550.pdf