초록
<P><B>Abstract</B></P> <P>Napier grass is a potential feedstock for biofuel production because of its strong adaptability and wide availability. Compositional analysis has been done on Napier grass which was collected from a local area of Taiwan. By comparing acid- and alkali-pretreatment, it was found that the alkali-pretreatment process is favorable for Napier grass. An overall glucose yield of 0.82g/g-glucose<SUB>total</SUB> can be obtained with the combination of alkali-pretreatment (2.5wt% NaOH, 8wt% sample loading, 121°C, and a reaction time of 40min) and enzymatic hydrolysis (40FPU/g-substrate). Semi-simultaneous saccharification fermentation (sSSF) was carried out, where enzymatic hydrolysis and ABE fermentation were operated in the same batch. It was found that after 24-h hydrolysis, followed by 96-h fermentation, the butanol and acetone concentrations reached 9.45 and 4.85g/L, respectively. The butanol yield reached 0.22g/g-sugar<SUB>glucose+xylose</SUB>. Finally, the efficiency of butanol production from Napier grass was calculated at 31%.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An 0.81g-glucose/g-glucose<SUB>total</SUB> is obtained in the enzymatic hydrolysate. </LI> <LI> Semi-simultaneous saccharification fermentation (sSSF) was employed. </LI> <LI> 9.45g/L butanol is obtained after a 24-h hydrolysis and 96-h fermentation. </LI> <LI> The butanol yield reached 0.22g/g-sugar<SUB>glucose+xylose.</SUB> </LI> <LI> The efficiency of butanol production from Napier grass was 31% </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>