초록
<P><B>Abstract</B></P> <P>The potential of bioH<SUB>2</SUB> production using waste bread via a two-stage process was examined. In the first stage, the waste bread was utilized by <I>Aspergillus awamori</I> and <I>Aspergillus oryzae</I> through solid state fermentation to generate glucoamylase and protease which were then utilized to hydrolyze the waste bread to produce the waste bread hydrolysate. The highest starch conversion of 96.6% and glucose yield of 0.521 g glucose/g waste bread were achieved within 24 h. In the second stage, the waste bread hydrolysate was utilized for bioH<SUB>2</SUB> production by <I>Biohydrogenbacterium</I> R3. The maximum H<SUB>2</SUB> yield of 103 mL H<SUB>2</SUB>/g waste bread could be obtained. The proposed two-stage bioprocess, which was able to increase the nutrient conversion efficiency and H<SUB>2</SUB> production from organic solid wastes, should be a promising way for bioH<SUB>2</SUB> production in industrial application.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-stage bioprocess for hydrogen production from bread was developed. </LI> <LI> Bread hydrolysate could be used to replace glucose for biohydrogen production. </LI> <LI> Enzymatic hydrolysis should be an effective pretreatment for bioH<SUB>2</SUB> production. </LI> <LI> The best hydrogen yield of 103 mL H<SUB>2</SUB>/g waste bread was achieved. </LI> </UL> </P>