BioChem - DOS

Production of value-added chemicals from glycerol using in vitro enzymatic cascades

Communications chemistry

Li, Zhong; Yan, Jinxin; Sun, Jinkai; Xu, Ping; Ma, Cuiqing; Gao, Chao

<P><B>Abstract</B><P>The large surplus of glycerol derived from the expanding biofuel industry raises economic and environmental concerns regarding disposal. In vitro synthetic biology is emerging as a useful biomanufacturing platform while the conversion of glycerol is rarely investigated. Here we develop a thermostable in vitro synthetic biosystem consisting of three enzymatic cascades for the biotransformation of glycerol into valuable chemicals with different degrees of reduction. Condensation of glycerol, phenol, and ammonium into l-tyrosine is achieved using four enzymes without the assistance of NAD<SUP>+</SUP>/NADH-related redox reactions. Production of chemicals with high degrees of reduction (e.g., optically pure l-lactate and d-lactate) is also verified through coupling with an NADH-regeneration system. The biotransformation of glycerol and ammonium into l-serine is achieved using four enzymes with self-sufficient NADH recycling.</P></P>

2018

Springer Science and Business Media LLC

2399-3669

1

1

pp.71

10.1038/s42004-018-0070-7

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART110158206

https://www.nature.com/articles/s42004-018-0070-7.pdf