BioChem - DOS

Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum

Process biochemistry

Zhu, B.H.; Tu, C.C.; Shi, H.P.; Yang, G.P.; Pan, K.H.

The effect of overexpression of endogenous delta-6 fatty acid desaturase gene (ER<DELTA>6FAD) on eicosapentaenoic acid (EPA) production and total lipid content was investigated in Phaeodactylum tricornutum. All three randomly selected transformants exhibited significant increase (47.66%) in their EPA content, which reached up to 38.101mg/g DW in algal strain D63. The total lipid content of all the three transformants was significantly higher (16.40-18.64%) than that of the wild-type strain. These findings suggested that the reaction catalyzed by ER<DELTA>6FAD is a limiting step for EPA biosynthesis, overexpression of endogenous ER<DELTA>6FAD gene is an effective method for improving EPA production in eukaryotic microalgae, and fatty acid metabolic pathway in microalgae can be genetically modified.

2017

Elsevier Applied Science

1359-5113

57

pp.43-49

10.1016/j.procbio.2017.03.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77884535

Diatom; EPA content; Total lipids; Δ6-desaturase