BioChem - DOS

Multi-objective optimization of media components for improved algae biomass, fatty acid and starch biosynthesis from Scenedesmus sp. ASK22 using desirability function approach

Renewable energy

Pandey, Ashutosh; Gupta, Aarti; Sunny, Arrabachala; Kumar, Sanjay; Srivastava, Sameer

<P><B>Abstract</B></P> <P>In this study, we sought to improve medium composition for enhanced biomass, lipid and starch content by using response surface methodology and composite desirability function approach for the heterotrophic cultivation of <I>Scenedesmus</I> sp. ASK22. Maximum biomass yield observed was 5.02 &plusmn; 0.1 g L<SUP>-1</SUP> and 4.67 &plusmn; 0.12 g L<SUP>-1</SUP> for individual and multi-response respectively. Lipid and starch accumulation for individual response and multi-response were found to be 30.72 &plusmn; 0.23 (% wt.) and 37.10 &plusmn; 2.71 (% wt.) and 29.46 &plusmn; 0.17 (% wt.) and 31.06 &plusmn; 0.17 (% wt.) respectively. The Lipid and starch productivity in BG11 medium increased to 13.38 and 28.59-fold for individual optimized conditions and 18.20 and 31.97-fold for multi-response optimized conditions respectively. These results propose <I>Scenedesmus</I> sp. ASK22 strain to be a potential candidate for bio-fuel production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Urea was found suitable for <I>Scenedesmus</I> sp. ASK22 as a nitrogen source. </LI> <LI> Medium was optimized by desirability function approach using <I>Scenedesmus</I> sp. ASK22. </LI> <LI> 18.2- and 31.97-fold increase were observed in lipid and starch production. </LI> <LI> 10.51-fold improvement was observed in <I>Scenedesmus</I> sp. ASK22 biomass. </LI> <LI> C18:1 account more than 50% of total fatty acid present in heterotrophic culture condition. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2020

Elsevier

0960-1481

150

pp.476-486

10.1016/j.renene.2019.12.095

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98945396

Scenedesmus sp. ASK22; Lipid; Starch; Optimization; Central composite design; Desirability function approach