초록
<P>Probiotic intervention is an effective strategy to alleviate oxidative stress-related diseases. Our previous studies found that <I>Lactiplantibacillus plantarum</I> NJAU-01 (NJAU-01) exhibited antioxidant effects in a d-galactose (d-gal)-induced aging mouse model. However, the underlying mechanism remains to be unveiled. This study was aimed to investigate the ameliorative effect and mechanism of NJAU-01 against oxidative stress induced by d-gal. The results showed that NJAU-01 could reverse the tendency of a slow body weight gain induced by d-gal. NJAU-01 relieved hepatic oxidative stress <I>via</I> increasing the hepatic total antioxidant capacity and antioxidant enzyme activities including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT). Moreover, the malondialdehyde (MDA) level was reversed after NJAU-01 supplementation. The proteomic results showed that there were 201 differentially expressed proteins (DEPs) between NJAU-01 and d-gal groups. NJAU-01 regulated the expressions of glutathione <I>S</I>-transferase Mu 5 (Gstm5), glutathione <I>S</I>-transferase P2 (Gstp2) and NADH dehydrogenase 1α subcomplex subunit 7 (Ndufa7) related to oxidative stress, and autophagy protein 5 (Atg5) and plasma alpha-l-fucosidase (Fuca2) involved in autophagy, <I>etc</I>. 16S rDNA sequencing results showed that NJAU-01 supplementation could regulate the gut microbiota dysbiosis induced by d-gal <I>via</I> increasing the relative abundances of the phylum <I>Firmicutes</I> and the genus <I>Lactobacillus</I> and reducing the relative abundances of the phylum <I>Bacteroidetes</I> and the genera <I>Lachnospiraceae</I>_NK4A136_group as well as <I>Prevotellaceae</I>_UCG-001, <I>etc.</I>. Spearman correlation analysis results showed that the altered gut microbiota composition had a significant correlation with antioxidant enzyme activities and the DEPs related to oxidative stress. Overall, NJAU-01 alleviated hepatic oxidative stress induced by d-gal <I>via</I> manipulating the gut microbiota composition and hepatic protein expression profile.</P>