BioChem - DOS

Fractionation of wheat straw by prehydrolysis, organosolv delignification and enzymatic hydrolysis for production of sugars and lignin

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Huijgen, W.J.J.; Smit, A.T.; de Wild, P.J.; den Uil, H.

Wheat straw was fractionated using a three-step biorefining approach: (1) aqueous pretreatment for hemicellulose prehydrolysis into sugars, (2) organosolv delignification, and (3) enzymatic cellulose hydrolysis into glucose. Prehydrolysis was applied to avoid degradation of hemicellulose sugars during organosolv delignification. Maximum xylose yield obtained was 67% or 0.17kg/kg straw (prehydrolysis: 175<SUP>o</SUP>C, 30min, 20mM H<SUB>2</SUB>SO<SUB>4</SUB>) compared to 4% in case of organosolv without prehydrolysis (organosolv: 200<SUP>o</SUP>C, 60min, 60% w/w aqueous ethanol). Prehydrolysis was found to reduce the lignin yield by organosolv delignification due to the formation of 'pseudo-lignin' and lignin recondensation during prehydrolysis. This reduction could partly be compensated by increasing the temperature of the organosolv delignification step. Prehydrolysis substantially improved the enzymatic cellulose digestibility from 49% after organosolv without prehydrolysis to 80% (20FPU/g substrate). Increasing the organosolv delignification temperature to 220<SUP>o</SUP>C resulted in a maximum enzymatic glucose yield of 93% or 0.36kg/kg straw.

2012

Elsevier Applied Science

0960-8524

114

pp.389-398

10.1016/j.biortech.2012.02.143

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62358095

https://publications.tno.nl/publication/34633546/l65f0R/w12004.pdf

Organosolv; Pretreatment; Enzymatic hydrolysis; Prehydrolysis; Biorefining