BioChem - DOS

Effects of linoleic acid and its degradation by-products on mesophilic hydrogen production using flocculated and granular mixed anaerobic cultures

International journal of hydrogen energy

Saady, N.M.C.; Chaganti, S.R.; Lalman, J.A.; Veeravalli, S.S.; Shanmugam, S.R.; Heath, D.D.

The effects of linoleic acid (LA (C18:2)) and its degradation by-products on hydrogen (H<SUB>2</SUB>) production were examined at 37 <SUP>o</SUP>C and an initial pH value of 5.0 using granular and flocculated mixed anaerobic cultures from the same source. In the flocculated cultures, the H<SUB>2</SUB> consumers were inhibited to a greater extent when compared to the granular cultures. The maximum H<SUB>2</SUB> yields were 2.52 +/- 0.2 and 1.9 +/- 0.2 mol mol<SUP>-1</SUP> glucose in the flocculated and granular cultures, respectively. The major long chain fatty acids (LCFAs) detected at which H<SUB>2</SUB> attained a maximum value were LA (750 mg L<SUP>-1</SUP>) and myristic acid (MA) (500 mg L<SUP>-1</SUP>). Bacteroides sp. dominated the granular cultures whereas Clostridium sp. and Bacillus spp. were the major microorganisms in the flocculated cultures. A flux balance analysis (FBA) revealed that homoacetogens consumed more H<SUB>2</SUB> in the granular when compared to the flocculated culture. A principal component analyses (PCA) showed the first three principal components accounted for approximately 76% and 68.5% of the total variability for the LA and its degradation by-products and the T-RFLP data sets, respectively.

2012

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

37

24

pp.18747-18760

10.1016/j.ijhydene.2012.09.065

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART64954896

Principal component analysis (PCA); Flux balance analysis; T-RFLP; Biohydrogen; Linoleic acid (LA (C18:2)); Anaerobic mixed culture