Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic Mid-Ocean Ridge
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic Mid-Ocean Ridge
학술지
Journal of agricultural and food chemistry
저자명
Vuoristo, Kiira S.; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus Ø.; Aarstad, Olav A.; Stokke, Runar; Steen, Ida H.; Hansen, Line Degn; Schü ller, Reidar B.; Aachmann, Finn L.; Horn, Svein J.; Eijsink, Vincent G. H.
초록
<P>Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, <I>Saccharina latissima</I>, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-<SMALL>D</SMALL>-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its <I>K</I>m and <I>k</I><SUB>cat</SUB> values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s<SUP>-1</SUP> respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.</P><BR>[FIG OMISSION]</BR>