초록
<P><B>Abstract</B></P> <P>A new β-glucosidase (Cel3B) was first isolated from cellulytic fungi, designated as PpCel3B. Although PpCel3B was classified to GH family 3 based on the homology sequence, PpCel3B had different biological functions in cellulose degradation and signaling molecules production. PpCel3B was constitutive and could form multiple soluble lignocellulose inducers for cellulase and hemicellulase synthesis via high tranglycosylation activity and new enzymatic activity. Moreover, PpCel3B showed apparent synergism with cellulases by removing several inhibitors. Supplementing low doses of PpCel3B (52μg/g substrate) increased saccharification efficiency of cellulase produced by <I>Trichoderma reesei</I> and <I>Penicillium piceum</I> by 15% and 35%, respectively on delignified corn stover. PpCel3B had important application in boosting cellulase yield and efficiency.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new β-glucosidase (PpCel3B) was firstly isolated and characterized from cellulytic fungi. </LI> <LI> PpCel3B had a very high transglycosylation activity to produce disaccharide derivatives. </LI> <LI> PpCel3B, with new enzymatic activity against xylotriose, could form hemicellulase inducers. </LI> <LI> PpCel3B play an important role in forming inducers for cellulase and hemicellulase synthesis. </LI> <LI> Supplementing low doses of PpCel3B could enhance different cellulase efficiency by 15–35%. </LI> </UL> </P>