BioChem - DOS

Cooperative growth of Geobacter sulfurreducens and Clostridium pasteurianum with subsequent metabolic shift in glycerol fermentation

Scientific reports

Moscoviz, Roman; de Fouché cour, Florence; Santa-Catalina, Gaë lle; Bernet, Nicolas; Trably, Eric

<P>Interspecies electron transfer is a common way to couple metabolic energy balances between different species in mixed culture consortia. Direct interspecies electron transfer (DIET) mechanism has been recently characterised with <I>Geobacter</I> species which couple the electron balance with other species through physical contacts. Using this mechanism could be an efficient and cost-effective way to directly control redox balances in co-culture fermentation. The present study deals with a co-culture of <I>Geobacter sulfurreducens</I> and <I>Clostridium pasteurianum</I> during glycerol fermentation. As a result, it was shown that <I>Geobacter sulfurreducens</I> was able to grow using <I>Clostridium pasteurianum</I> as sole electron acceptor. <I>C. pasteurianum</I> metabolic pattern was significantly altered towards improved 1,3-propanediol and butyrate production (+37% and +38% resp.) at the expense of butanol and ethanol production (&#x2212;16% and &#x2212;20% resp.). This metabolic shift was clearly induced by a small electron uptake that represented less than 0.6% of the electrons consumed by <I>C. pasteurianum</I>. A non-linear relationship was found between <I>G. sulfurreducens</I> growth (<I>i.e</I> the electrons transferred between the two species) and the changes in <I>C. pasteurianum</I> metabolite distribution. This study opens up new possibilities for controlling and increasing specificity in mixed culture fermentation.</P>

2017

Nature Publishing Group

cc-by

2045-2322

7

pp.44334

10.1038/srep44334

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77535815

https://www.nature.com/articles/srep44334.pdf