초록
<P><B>Abstract</B></P> <P>This article presents a study on identification, cultivation and characterisation of microalgal strains from the coastal waters of the Pearl River Delta in Guangdong, China. Thirty-seven identified strains belong to the families: <I>Chlorellaceae</I>, <I>Scotiellocystoidaceae</I>, <I>Scenedesmaceae</I>,<I>Selenastraceae</I>,<I>Micractiniaceae</I>, <I>Coccomyxaceae</I>, <I>Trebouxiaceae</I> and <I>Chlorococcaceae</I>. Of isolated strains, <I>Hindakia</I> PKUAC 169 was selected for lipid induction using two methods: nitrogen starvation and salt stress. After derivatisation of algal lipids through <I>in situ</I> transesterification, lipid profiles of the alga under the two methods were analysed. The results have shown that both lipid yield and fatty acid profiles vary with the methods. Of the two tested methods of inducing lipid production, salt stress yielded three-fold higher lipid productivity than nitrogen starvation. The lipids are predominantly composed of C14–C18 fatty acids, which are favourable for biodiesel production. Moreover, the content of polyunsaturated fatty acids was below the limit of 12% set by EN14214 biodiesel standard.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Thirty-seven algal strains were isolated from coastal waters of Pearl River Delta. </LI> <LI> <I>Hindakia</I> strain native to Pearl River Delta was selected for FAME production. </LI> <LI> Two methods of lipid induction were used: salt stress and nitrogen starvation. </LI> <LI> Salt stress yields three-fold higher lipid productivity than nitrogen starvation. </LI> </UL> </P>