BioChem - DOS

Construction of an efficient Escherichia coli whole-cell biocatalyst for D-mannitol production

Journal of bioscience and bioengineering

Reshamwala, S.M.S.; Pagar, S.K.; Velhal, V.S.; Maranholakar, V.M.; Talangkar, V.G.; Lali, A.M.

Mannitol is a six carbon sugar alcohol that finds applications in the pharmaceutical and food industries. A novel Escherichia coli strain capable of converting d-glucose to d-mannitol has been constructed, wherein native mannitol-1-phosphate dehydrogenase (MtlD) and codon-optimized Eimeria tenella mannitol-1-phosphatase (M1Pase) have been overexpressed. Codon-optimized Pseudomonas stutzeri phosphite dehydrogenase (PtxD) was overexpressed for cofactor (NADH) regeneration with the concomitant oxidation of phosphite to phosphate. Whole-cell biotransformation using resting cells in a medium containing d-glucose and equimolar sodium phosphite resulted in d-mannitol yield of 87 mol%. Thus, production of an industrially relevant biochemical without using complex media components and elaborate process control mechanisms has been demonstrated.

2014

Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science

1389-1723

1347-4421

118

6

pp.628-631

10.1016/j.jbiosc.2014.05.004

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70685925

Cofactor regeneration; Biotransformation; Resting cells; d-Mannitol production; Whole-cell biocatalyst