BioChem - DOS

Efficient enzymatic production of rebaudioside A from stevioside

Bioscience, biotechnology, and biochemistry

Wang, Yu; Chen, Liangliang; Li, Yan; Li, Yangyang; Yan, Ming; Chen, Kequan; Hao, Ning; Xu, Lin

<P><B>Abstract</B><P>Stevioside and rebaudioside A are the chief diterpene glycosides present in the leaves of Stevia rebaudiana. Rebaudioside A imparts a desirable sweet taste, while stevioside produces a residual bitter aftertaste. Enzymatic synthesis of rebaudioside A from stevioside can increase the ratio of rebaudioside A to stevioside in steviol glycoside products, providing a conceivable strategy to improve the organoleptic properties of steviol glycoside products. Here, we demonstrate the efficient conversion of stevioside to rebaudioside A by coupling the activities of recombinant UDP-glucosyltransferase UGT76G1 from S. rebaudiana and sucrose synthase AtSUS1 from Arabidopsis thaliana. The conversion occurred via regeneration of UDP-glucose by AtSUS1. UDP was applicable as the initial material instead of UDP-glucose for UDP-glucose recycling. The amount of UDP could be greatly reduced in the reaction mixture. Rebaudioside A yield in 30 h with 2.4 mM stevioside, 7.2 mM sucrose, and 0.006 mM UDP was 78%.</P></P>

2016

Informa UK (TaylorFrancis)

0916-8451

1347-6947

80

1

pp.67-73

10.1080/09168451.2015.1072457

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75996124

https://academic.oup.com/bbb/article-pdf/80/1/67/36807503/bbb0067.pdf