초록
<P><B>Abstract</B></P> <P>Simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated <I>Kluyveromyces</I> sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5–15% solid loading and 6–12FPUg<SUP>−1</SUP> substrate enzyme loading for different time intervals 0–72h at 42°C. Maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from <I>Aspergillus terreus</I> was 23.23, 18.29 and 17.91mgmL<SUP>−1</SUP> at 60h with 10% solid load and 9FPUg<SUP>−1</SUP> substrate enzyme loading. Tween 80 1% (v/v) enhanced the ethanol yield by 8.39%, 9.26% and 8.14% in rice straw, wheat straw and sugarcane bagasse, respectively. External supplementation of β-glucosidase to the crude as well commercial cellulases produced maximum theoretical ethanol yield of 71.76%, 63.77%, 57.15% and 84.56%, 72.47%, 70.55% from rice straw, wheat straw and sugarcane bagasse, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Assessment of ethanol production from delignified lignocellulosic biomass by SSF. </LI> <LI> SSF at high solid loadings by a newly isolated thermotolerant yeast strain. </LI> <LI> Supplementation of β-glucosidase ensured better cellobiose conversion to glucose. </LI> </UL> </P>