BioChem - DOS

Enzymatic saccharification of acid-alkali pretreated sugarcane bagasse using commercial enzyme preparations

Journal of chemical technology and biotechnology

Giese, Ellen C.; Pierozzi, Maurí cio; Dussá n, Kelly J.; Chandel, Anuj K.; da Silva, Silvio S.

<P><B>Abstract</B></P><P><B>Background</B></P><P><B>The bioconversion of lignocellulosic biomass to second generation ethanol has the potential to replace some fossil fuels. Sugarcane bagasse (SB) is a readily available feedstock for ethanol production. The aim of this work was to study the enzymatic saccharification of consecutively acid&ndash;alkali pretreated SB using different commercial enzyme preparations.</B></P><P><B>Results</B></P><P><B>Under the most favourable enzymatic hydrolysis conditions, a maximum sugar recovery of 0.89&thinsp;g of reducing sugars g<SUP>&#8208;1</SUP> acid&ndash;alkali pretreated SB was obtained. Scanning electron microscopy (SEM) revealed the structural disruption in the SB morphology after acid&ndash;alkali pretreatment followed by enzymatic hydrolysis.</B></P><P><B>Conclusion</B></P><P><B>These results improve research strategies and show the susceptibility of SB under different enzymatic actions. This is thought to be the first report on utilization of cellulase AF28918 for SB saccharification into fermentable sugars.</B></P>

2013

John Wiley Sons, Ltd

0268-2575

1097-4660

88

7

pp.1266-1272

10.1002/jctb.3968

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66386327

sugarcane bagasse; acid&#x2013; alkali pretreatment; enzymatic hydrolysis; cellulase AF28918; scanning electron microscopy;