Pensupa, Nattha; Jin, Meng; Kokolski, Matt; Archer, David B.; Du, Chenyu
초록
<▼1><P><B>Highlights</B></P><P>•<P>A solid state fungal fermentation strategy converting wheat straw to hydrolysate.</P>•<P>A biological pre-treatment of wheat straw by culturing <I>A. niger</I> on wheat straw.</P>•<P>24.0 ± 1.76 U/g cellulase was produced using wheat straw as the main substrate.</P>•<P>The fungal extract was used to hydrolyze the fermented wheat straw.</P>•<P>19% higher hydrolysis efficiency using freshly-prepared fungal extract than Ctec2.</P></P></▼1><▼2><P>This paper reports a solid-state fungal fermentation-based pre-treatment strategy to convert wheat straw into a fermentable hydrolysate. <I>Aspergillus niger</I> was firstly cultured on wheat straw for production of cellulolytic enzymes and then the wheat straw was hydrolyzed by the enzyme solution into a fermentable hydrolysate. The optimum moisture content and three wheat straw modification methods were explored to improve cellulase production. At a moisture content of 89.5%, 10.2 ± 0.13 U/g cellulase activity was obtained using dilute acid modified wheat straw. The addition of yeast extract (0.5% w/v) and minerals significantly improved the cellulase production, to 24.0 ± 1.76 U/g. The hydrolysis of the fermented wheat straw using the fungal culture filtrate or commercial cellulase Ctec2 was performed, resulting in 4.34 and 3.13 g/L glucose respectively. It indicated that the fungal filtrate harvested from the fungal fermentation of wheat straw contained a more suitable enzyme mixture than the commercial cellulase.</P></▼2>
발행연도
2013
발행기관
Elsevier Applied Science ;, Elsevier Science Pub. Co