초록
Palladium nanoparticles (PdNPs) were synthesized from PdCl<SUB>2</SUB> using Coriandrum sativum leaf extract. The transmission electron microscopy (TEM) images confirm that the formation of PdNPs was mainly spherical in shape, with an average size of 87nm. The influence of the PdCl<SUB>2</SUB> and synthesized PdNPs on fermentative hydrogen production from glucose using Enterobacter cloacae and mixed culture was evaluated. In PdCl<SUB>2</SUB> supplemented experiments, the hydrogen yields of E. cloacae and mixed culture were 1.39+/-0.07 and 2.11+/-0.11mol H<SUB>2</SUB>/mol glucose, respectively, with 5.0mg/L of PdCl<SUB>2</SUB>. The resulting hydrogen yield (P<0.05) was lower than that of the control experiment (without supplementation), due to the soluble metabolites shift. However, the highest hydrogen yields of E. cloacae and mixed culture were 1.48+/-0.04 and 2.48+/-0.09mol H<SUB>2</SUB>/mol glucose, respectively at 5.0mg/L of PdNPs supplementation. The enhancement of biohydrogen production using mixed culture was significantly higher than that of E. cloacae as the same concentration of PdNPs.