초록
<P><B>Abstract</B></P> <P>Two-stage process of dark fermentation (DF) and photo fermentation (PF), using fruit and vegetable waste (FVW) and cheese whey powder (CWP), was used as an approach to enhance the hydrogen (H<SUB>2</SUB>) production. FVW and CWP at C/N ratios of 34, 39, 60, 71 and 82 were tested as substrates for DF. Dilution (1:2, 1:5, 1:10) of the DF effluents was used as a coupling strategy. DF effluents with low-butyrate and high lactate concentrations were obtained as a function of an increased C/N ratio, which results in high H<SUB>2</SUB> production during the PF. Maximum overall H<SUB>2</SUB> yields of 793.7 and 695.4 mLH<SUB>2</SUB>/gChemical Oxygen Demand (COD) were obtained using a 1:10 dilution, at a C/N ratio of 60 and 70, respectively. These H<SUB>2</SUB> yields were higher than those obtained with the individual processes. The C/N ratio at the DF stage regulate not only H<SUB>2</SUB> production but also the distribution and concentrations of by-products. These metabolites, in turn, control the H<SUB>2</SUB> production during the PF. Predominant microbial population for both processes (DF: C/N = 34 <I>Acetobacter lovaniensis</I>, <I>Clostridium butyricum</I>; C/N = 39 <I>C. butyricum, Enterobacter</I> sp<I>, Bifidobacterium</I>; C/N = 82 <I>Lactobacillus casei</I>; PF: <I>Rhodopseudomonas palustris</I>) were in accordance with the final metabolic products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced H<SUB>2</SUB> production by dark-photo (DF-PF) fermentations was demonstrated. </LI> <LI> Fruit and vegetable waste and cheese whey as substrate allows waste removal and H<SUB>2</SUB> production. </LI> <LI> Increased C/N ratio reduces H<SUB>2</SUB> yield at DF, but increase the H<SUB>2</SUB> production at PF. </LI> <LI> DF effluents with high lactate concentrations promotes high H<SUB>2</SUB> production at PF. </LI> <LI> Microbial population for both DF, PF in accordance with the final metabolic products. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>