초록
<P><B>Abstract</B></P><P>Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57–74.3% of holocellulose fraction. This study was aimed at utilizing SMS of <I>Pleurotus florida</I> for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of <I>Trichoderma</I> and <I>Aspergillus</I> under solid‐state fermentation (SSF). SMS of <I>P. florida</I> extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, <I>Trichoderma longibrachiatum</I> A‐01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while <I>Aspergillus aculeatus</I> C‐08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2‐, 4.5‐, 4.1‐, and 5.0‐fold as compared with crude extract. The results reveal that SMS from <I>P. florida</I> could be a potential and cost‐effective substrate for production of cellulolytic enzymes from <I>T. longibrachiatum</I> A‐01 and <I>A. aculeatus</I> C‐08.</P>