BioChem - DOS

De novo biosynthesis of caffeic acid from glucose by engineered Saccharomyces cerevisiae

ACS Synthetic biology

Li, Yuanzi; Mao, Jiwei; Liu, Quanli; Song, Xiaofei; Wu, Yuzhen; Cai, Miao; Xu, Haijin; Qiao, Mingqiang

<P>Caffeic acid is a plant phenolic compound possessing extensive pharmacological activities. Here, we identified that <I>p</I>-coumaric acid 3-hydroxylase from <I>Arabidopsis thaliana</I> was capable of hydroxylating <I>p</I>-coumaric acid to form caffeic acid in <I>Saccharomyces cerevisiae</I>. Then, we introduced a combined caffeic acid biosynthetic pathway into <I>S. cerevisiae</I> and obtained 0.183 mg L<SUP>-1</SUP> caffeic acid from glucose. Next we improved the tyrosine biosynthesis in <I>S. cerevisiae</I> by blocking the pathway flux to aromatic alcohols and eliminating the tyrosine-induced feedback inhibition resulting in caffeic acid production of 2.780 mg L<SUP>-1</SUP>. Finally, the medium was optimized, and the highest caffeic acid production obtained was 11.432 mg L<SUP>-1</SUP> in YPD medium containing 4% glucose. This study opens a route to produce caffeic acid from glucose in <I>S. cerevisiae</I> and establishes a platform for the biosynthesis of caffeic acid derived metabolites.</P><BR>[FIG OMISSION]</BR>

2020

American Chemical Society

2161-5063

9

4

pp.756-765

10.1021/acssynbio.9b00431.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART99580877

https://doi.org/10.1021/acssynbio.9b00431.s001

Saccharomyces cerevisiae; caffeic acid; p-coumaric acid 3-hydroxylase; metabolic engineering; de novo