Study on the directional breeding of high glutamic-acid production strain GTS190 and the control on its fermentation

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논문

Study on the directional breeding of high glutamic-acid production strain GTS190 and the control on its fermentation

학술지

Advanced materials research : AMR

저자명

Li, Zhi Hua; Chen, Ning

초록

<P>Using Brevibacterium flavum CN1021 as starting strain, the L-glutamate producing strain was directionally bred with protoplast UV mutagenesis, UV mutagenesis and DES mutagenesis. Through shake-tube preliminary screening, shake-flask re-screening, genetic markers verification and continuous passage, an L-glutamate temperature-sensitive mutant strain GTS190 (Ts+Omr+GluHXr+ SGr+FEAr+AECr+Sucg) was screened finally. It was fermented for 36h in a vessel (30L) feeding by batches, in which glutamic acid production reached 195g/L and sugar and acid conversion rate was 66%.</P>

발행연도

2012

발행기관

Trans Tech Publications, Ltd.

ISSN

1022-6680

ISSN

1662-8985

권

573

페이지

pp.1029-1033