초록
The effect of corn stover pretreatment on glucose quantitation in hydrolysate using Raman spectroscopy is evaluated. Dilute sulfuric-acid pretreatment results in a 20mgmL<SUP>-1</SUP> glucose limit of detection in hydrolysate. Soaking in aqueous ammonia pretreatment produces a 4mgmL<SUP>-1</SUP> limit of detection. Water, ethanol or hexane extraction of corn stover reduces the spectral background that limits glucose detection in dilute acid hydrolysate. Additionally, a Raman spectroscopy multi-peak fitting method is presented to simultaneously measure glucose and xylose concentration in hydrolysate. This method yields a 6.1% average relative standard error at total saccharide concentrations above 45mgmL<SUP>-1</SUP>. When only cellulase is present, glucose and xylose yield were measured by Raman spectroscopy to be 32+/-4 and 7.0+/-0.8mgmL<SUP>-1</SUP>, respectively. When both cellulase and hemicellulase were present, xylose yield increased to 18.0+/-0.5mgmL<SUP>-1</SUP>. Enzymatic or colorimetric assays confirmed the validity of the Raman spectroscopy results.