BioChem - DOS

Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

Metabolic engineering

Borodina, Irina; Kildegaard, Kanchana R.; Jensen, Niels B.; Blicher, Thomas H.; Maury, Jé rô me; Sherstyk, Svetlana; Schneider, Konstantin; Lamosa, Pedro; Herrgå rd, Markus J.; Rosenstand, Inger; Ö berg, Fredrik; Forster, Jochen; Nielsen, Jens

<P><B>Abstract</B></P> <P>Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO<SUB>2</SUB> emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing <I>Saccharomyces cerevisiae</I> as an efficient cell factory for high-level production of 3HP, we identified the &beta;-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for <I>de novo</I> biosynthesis of &beta;-alanine and its subsequent conversion into 3HP using a novel &beta;-alanine-pyruvate aminotransferase discovered in <I>Bacillus cereus</I>. The final strain produced 3HP at a titer of 13.7&plusmn;0.3gL<SUP>&minus;1</SUP> with a 0.14&plusmn;0.0C-molC-mol<SUP>&minus;1</SUP> yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 3HP is a potential source of acrylic plastics and biodegradable polyesters. </LI> <LI> We report high-level production of 3HP from a sugar feedstock by yeast. </LI> <LI> A <I>de novo</I> route for 3HP biosynthesis via &beta;-alanine was engineered. </LI> <LI> Using yeast as the cell factory enables a more economical low pH process. </LI> </UL> </P>

2015

Elsevier

cc-by

1096-7176

1096-7184

27

pp.57-64

10.1016/j.ymben.2014.10.003

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71056619

Biosustainable acrylics; 3-hydroxypropionic acid; Saccharomyces cerevisiae; β-alanine; β-alanine-pyruvate aminotransferase