BioChem - DOS

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Dai, Zhongxue; Gu, Honglian; Zhang, Shangjie; Xin, Fengxue; Zhang, Wenming; Dong, Weiliang; Ma, Jiangfeng; Jia, Honghua; Jiang, Min

<P><B>Abstract</B></P> <P>The aim of this study was to metabolically construct <I>Saccharomyces cerevisiae</I> for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from <I>Pichia pastoris</I> into the chromosome of <I>S. cerevisiae</I>, the recombinant showed 1.04g/L consumption of methanol and 3.13% increase of cell growth (OD<SUB>600</SUB>) when using methanol as the sole carbon source. Moreover, 0.26g/L of pyruvate was detected in the fermentation broth. The supplementation of 1g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>S. cerevisiae</I> was firstly genetically constructed to achieve direct methanol conversion for pyruvate production. </LI> <LI> 1.04g/L of methanol was consumed when using methanol alone. </LI> <LI> 3.13% increase of cell growth and 0.26g/L of pyruvate was detected. </LI> <LI> Supplementation of yeast extract led to further improvement in cell growth and methanol consumption. </LI> </UL> </P>

2017

Elsevier

0960-8524

245

2

pp.1407-1412

10.1016/j.biortech.2017.05.100

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78806243

Methanol; Non-methylotrophy; S. cerevisiae; Synthetic biology; Pyruvate