Production of bacterial cellulose hydrogels with tailored crystallinity from Enterobacter sp. FY-07 by the controlled expression of colanic acid synthetic genes
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Production of bacterial cellulose hydrogels with tailored crystallinity from Enterobacter sp. FY-07 by the controlled expression of colanic acid synthetic genes
<P><B>Abstract</B></P> <P>Hydrogels exhibit smart three-dimensional networks and extraordinary water-absorbing ability. To improve the water-holding capacity of bacterial cellulose hydrogels, the expression of a biosynthetic gene cluster of colanic acid, a water-soluble polysaccharide, was induced in <I>Enterobacter</I> sp. FY-07, which produces an abundance of bacterial cellulose hydrogel under aerobic and anaerobic fermentation conditions. The results indicated that <I>in situ</I> modified bacterial cellulose hydrogels with different crystallinities, rheological properties and water-holding capacities were produced by cultivating the engineered strain <I>Enterobacter</I> sp. FY-07::<I>tac</I> under different inducing conditions. The water-holding capacity of the modified bacterial cellulose hydrogel was enhanced by more than 1.7 fold compared to the hydrogel produced by <I>Enterobacter</I> sp. FY-07, and the networks of the modified bacterial cellulose hydrogel were densified but still clear. These results suggest that this <I>in situ</I> modification strategy endows bacterial cellulose hydrogels with improved properties and potentially expands their applications in biomedical fields and the food industry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genes of colanic acid were induced in cellulose produced by <I>Enterobacter</I> sp. FY-07. </LI> <LI> Co-production of BC and modifiers impart MBC hydrogels fine microstructures. </LI> <LI> Modifying cellulose hydrogels alters the crystallinity and rheological properties. </LI> <LI> Hydrogel water-holding capacity and network structure were greatly improved. </LI> </UL> </P>