초록
<P><B>Background</B></P><P>Propane (C<SUB>3</SUB>H<SUB>8</SUB>) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel, in addition to existing global markets and infrastructure for storage, distribution and utilization in a wide range of applications. Consequently, propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. This study focuses on the construction and evaluation of alternative microbial biosynthetic pathways for the production of renewable propane. The new pathways utilize CoA intermediates that are derived from clostridial-like fermentative butanol pathways and are therefore distinct from the first microbial propane pathways recently engineered in <I>Escherichia coli</I>.</P><P><B>Results</B></P><P>We report the assembly and evaluation of four different synthetic pathways for the production of propane and butanol, designated a) <I>atoB</I>-<I>adhE2</I> route, b) <I>atoB</I>-<I>TPC7</I> route, c) <I>nphT7</I>-<I>adhE2</I> route and d) <I>nphT7</I>-<I>TPC7</I> route. The highest butanol titres were achieved with the <I>atoB-adhE2</I> (473 ± 3 mg/L) and <I>atoB-TPC7</I> (163 ± 2 mg/L) routes. When aldehyde deformylating oxygenase (ADO) was co-expressed with these pathways, the engineered hosts also produced propane. The <I>atoB-TPC7-ADO</I> pathway was the most effective in producing propane (220 ± 3 μg/L). By (i) deleting competing pathways, (ii) including a previously designed ADO<SUB>A134F</SUB> variant with an enhanced specificity towards short-chain substrates and (iii) including a ferredoxin-based electron supply system, the propane titre was increased (3.40 ± 0.19 mg/L).</P><P><B>Conclusions</B></P><P>This study expands the metabolic toolbox for renewable propane production and provides new insight and understanding for the development of next-generation biofuel platforms. In developing an alternative CoA-dependent fermentative butanol pathway, which includes an engineered ADO variant (ADO<SUB>A134F</SUB>), the study addresses known limitations, including the low bio-availability of butyraldehyde precursors and poor activity of ADO with butyraldehyde.</P><P><BR>>[FIG OMISSION]</BR></P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13068-015-0231-1) contains supplementary material, which is available to authorized users.</P>