BioChem - DOS

Acrylamide production using encapsulated nitrile hydratase from Pseudonocardia thermophila in a sol-gel matrix

Journal of molecular catalysis. B, Enzymatic

Martinez, S.; Kuhn, M.L.; Russell, J.T.; Holz, R.C.; Elgren, T.E.

The cobalt-type nitrile hydratase from Pseudonocardia thermophila JCM 3095 (PtNHase) was successfully encapsulated in tetramethyl orthosilicate sol-gel matrices to produce a PtNHase:sol-gel biomaterial. The PtNHase:sol-gel biomaterial catalyzed the conversion of 600mM acrylonitrile to acrylamide in 60min at 35<SUP>o</SUP>C with a yields of >90%. Treatment of the biomaterial with proteases confirmed that the catalytic activity is due to the encapsulated enzyme and not surface bound NHase. The biomaterial retained 50% of its activity after being used for a total of 13 consecutive reactions for the conversion of acrylonitrile to acrylamide. The thermostability and long-term storage of the PtNHase:sol-gel are substantially improved compared to the soluble NHase. Additionally, the biomaterial is significantly more stable at high concentrations of methanol (50% and 70%, v/v) as a co-solvent for the hydration of acrylonitrile than native PtNHase. These data indicate that PtNHase:sol-gel biomaterials can be used to develop new synthetic avenues involving nitriles as starting materials given that the conversion of the nitrile moiety to the corresponding amide occurs under mild temperature and pH conditions.

2014

Elsevier

1381-1177

1873-3158

100

pp.19-24

10.1016/j.molcatb.2013.11.014

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68536196

Nitrile hydratase; Sol-gels; Enzyme immobilization; Biomaterials; Cobalt