BioChem - DOS

Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Mohd Yusoff, Mohd Zulkhairi; Akita, Hironaga; Hassan, Mohd Ali; Fujimoto, Shinji; Yoshida, Masaru; Nakashima, Nobutaka; Hoshino, Tamotsu

<P><B>Abstract</B></P> <P>Acetoin is used in the biochemical, chemical and pharmaceutical industries. Several effective methods for acetoin production from petroleum-based substrates have been developed, but they all have an environmental impact and do not meet sustainability criteria. Here we describe a simple and efficient method for acetoin production from oil palm mesocarp fiber hydrolysate using engineered <I>Escherichia coli</I>. An optimization of culture conditions for acetoin production was carried out using reagent-grade chemicals. The final concentration reached 29.9gL<SUP>&minus;1</SUP> with a theoretical yield of 79%. The optimal pretreatment conditions for preparing hydrolysate with higher sugar yields were then determined. When acetoin was produced using hydrolysate fortified with yeast extract, the theoretical yield reached 97% with an acetoin concentration of 15.5gL<SUP>&minus;1</SUP>. The acetoin productivity was 10-fold higher than that obtained using reagent-grade sugars. This approach makes use of a compromise strategy for effective utilization of oil palm biomass towards industrial application.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 29.9gL<SUP>&minus;1</SUP> acetoin were produced from 70gL<SUP>&minus;1</SUP> glucose under optimized conditions. </LI> <LI> Enzymatic hydrolysis efficiency was enhanced using the hot compressed water method. </LI> <LI> Theoretical yield for acetoin reached 97% from hydrolysate plus yeast extract. </LI> <LI> Acetoin productivity of 0.64gL<SUP>&minus;1</SUP> h<SUP>&minus;1</SUP> was greater than from reagent-grade sugars. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

publisher-specific-oa

0960-8524

245

1

pp.1040-1048

10.1016/j.biortech.2017.08.131

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78626224

http://manuscript.elsevier.com/S0960852417314451/pdf/S0960852417314451.pdf

Acetoin; Escherichia coli; Hydrolysate; Mineral source; Oil palm mesocarp fiber